DAIMON 2 project has worked out a set of state-of-art, time and cost-saving methodologies for the detection,
impact analysis and monitoring of dumped munitions:
1. Methods for munitions detection’ and identification
2. Chemical methods for detecting warfare agents (parent compounds and degradation products) in water, sediments and biota
3. Biological methods for detecting effects of toxic warfare agents from chemical and conventional munitions in marine organisms (vertebrates, invertebrates)
4. Guidelines for additional measurements e.g. addressing habitat condition
5. Guidelines for data analysis and assessment
They are widely acknowledged in the international scientific community and already applied by members of the DAIMON network.
impact analysis and monitoring of dumped munitions:
1. Methods for munitions detection’ and identification
2. Chemical methods for detecting warfare agents (parent compounds and degradation products) in water, sediments and biota
3. Biological methods for detecting effects of toxic warfare agents from chemical and conventional munitions in marine organisms (vertebrates, invertebrates)
4. Guidelines for additional measurements e.g. addressing habitat condition
5. Guidelines for data analysis and assessment
They are widely acknowledged in the international scientific community and already applied by members of the DAIMON network.
List of finalised DAIMON SOPs:
- Sediment sampling with gravity corer
- Sediment sampling with ROV
- Sediment sampling with Box corer and Van Veen grab
- Sampling safety procedures
- People safety procedures
- Acetylcholinesterase inhibition
- Catalase activity
- Homogenisation of fish liver and mussel digestive gland tissues
- Homogenisation of fish muscle and mussel gill tissues
- Glutathione peroxidase activity
- Glutathione reductase activity
- Lipid peroxidation
- Mussel caging approach
- Sampling of mussels
- Superoxide dismutase activity
- Glutathione-S-transferase activity
- Lysosomal membrane stability
- Data analysis and assessment (under review)
- Sampling of wild fish
- Fulton's condition factor
- Zebrafish embryo acute toxicity test (FET)
- Comet assay (applied to zebrafish embryos)
- Extraction and analysis of explosives and metabolites in fish bile via HPLC-QQQ-MS
- Externally visible fish diseases
- Fish liver hispathology
- Macroscopic liver neoplasms
- Hepatosomatic index
- Fish caging approach
- Micronucleus assay (MN)
- Chemical analysis of sea-dumped chemical warfare agents in sediment, and pore water
- Chemical analysis of degradation products of phenylarsenic CWAs in fish
- Chemical analysis of degradation products of phenylarsenic CWAs in mussel
- Chemical analysis of sea-dumped explosives in sediment (under review)