DAIMON Project

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    • NEWS & EVENTS
    • DSS Trainings
    • Partners
    • MEDIA
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  • MUNITIONS
    • Conventional munitions
    • Wrecks
    • Chemical Munitions
  • MANAGEMENT
    • Decision Support System (DSS)
    • Munitions Database
    • Catalogue of Munitions' Types
    • Standard Operation Procedures (SOPs)
    • EcoTox Toolbox
  • Intranet
  • Contact
  • ABOUT
    • NEWS & EVENTS
    • DSS Trainings
    • Partners
    • MEDIA
    • Related projects
  • MUNITIONS
    • Conventional munitions
    • Wrecks
    • Chemical Munitions
  • MANAGEMENT
    • Decision Support System (DSS)
    • Munitions Database
    • Catalogue of Munitions' Types
    • Standard Operation Procedures (SOPs)
    • EcoTox Toolbox
  • Intranet
  • Contact

​Standard Operation Procedures (SOPs)

DAIMON project has worked out a set of state-of-art, time and cost-saving methodologies for the detection,
impact analysis and monitoring of dumped munitions:


1. Methods for munitions detection’ and identification
2. Chemical methods for detecting warfare agents (parent compounds and degradation products) in water, sediments and biota 
3. Biological methods for detecting effects of toxic warfare agents from chemical and conventional munitions in marine organisms (vertebrates, invertebrates)
4. Guidelines for additional measurements e.g. addressing habitat condition
5. Guidelines for data analysis and assessment

They are widely acknowledged in the international scientific community and already applied by members of the DAIMON network.
DAIMON Standard Operating Procedures (SOPs)
ALL finalised SOPs (30.11.2020)
List of finalised DAIMON SOPs:
  1. Sediment sampling with gravity corer
  2. Sediment sampling with ROV
  3. Sediment sampling with Box corer and Van Veen grab
  4. Sampling safety procedures
  5. People safety procedures
  6. Acetylcholinesterase inhibition
  7. Catalase activity
  8. Homogenisation of fish liver and mussel digestive gland tissues
  9. Homogenisation of fish muscle and mussel gill tissues
  10. Glutathione peroxidase activity
  11. Glutathione reductase activity
  12. Lipid peroxidation
  13. Mussel caging approach
  14. Sampling of mussels
  15. Superoxide dismutase activity
  16. Glutathione-S-transferase activity
  17. Lysosomal membrane stability
  18. Data analysis and assessment (under review)
  19. Sampling of wild fish
  20. Fulton's condition factor 
  21. Zebrafish embryo acute toxicity test (FET) 
  22. Comet assay (applied to zebrafish embryos)
  23. Extraction and analysis of explosives and metabolites in fish bile via HPLC-QQQ-MS
  24. Externally visible fish diseases
  25. Fish liver hispathology
  26. Macroscopic liver neoplasms 
  27. Hepatosomatic index 
  28. Fish caging approach (under review)
  29. Micronucleus assay (MN)
  30. Chemical analysis of sea-dumped chemical warfare agents in sediment, and pore water (under review)
  31. Chemical analysis of degradation products of phenylarsenic CWAs in fish
  32. Chemical analysis of degradation products of phenylarsenic CWAs in mussel​
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